Amylase HT – 30K (food grade)

An Enzyme Supplies high temperature amylase for the brewing & distilling industries


Amylase HT-30K is a high temperature α-amylase produced by submerged fermentation of Bacillus licheniformis. The enzyme is an endo-amylase, hydrolyzing 1,4-α-glucosidic linkages in amylase & amylopectin at random, producing dextrins of different chain lengths & oligosaccharides. This results in a rapid decrease in the viscosity of starch solutions.

Typical Characteristics

Activity minimum 30,000 μ/ml (where 1μ/ml is defined as the amount of enzyme in 1ml enzyme solution to liquefy 1mg of soluble starch / minute at pH 6.0 & 70°C)
Appearance – brown liquid
Bulk density 1.2-1.25 g/ml


Amylase HT-30K is available in bulk (1000Kg IBC’s), and in 25Kg ‘non-toxic’ plastic barrels


To maintain optimum activity, Amylase HT-30K should be stored at moderate temperatures (below 25 deg C), in the original containers and with the lid closed. When stored at temperatures below 25 deg C, the activity loss will be less than 10% in 6 months. At temperatures below 5 deg C, the activity loss will be less than 10% in 1 year.


Inhalation of aerosols from liquid enzymes should be avoided. Open in a well-ventilated environment. In case of contact with skin/eyes, promptly rinse with water for at least 15 minutes. Clean any spillages with low-pressure water hoses, avoiding the formation of aerosols. For more detailed information please refer to the Material Safety Data Sheet for this product


Amylase HT-30K has an optimum pH range of 5.5-7.0 (effective range 5.0-8.0). The enzyme is very heat stable, active over a temperature range from 90°C to 100°C, with an optimum performance at 90 to 95°C. When used for continuous liquefaction in steam jet cookers or similar, Amylase HT-30K can operate at temperatures of between 105°C to 110°C. Calcium ion addition at a rate of 50mg/kg - 100mg/kg is recommended to ensure optimal enzyme thermostability.

Application Scope

Amylase HT-30K is widely used for high temperature liquefaction applications in the starch, alcohol, brewing, & ethanol industries. The enzyme is also a highly effective desizing agent for the textiles industry.

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We have been supplied by Enzyme Supplies for over 3 years and have found their material and service very satisfactory. On one occasion they helped to supply at very short notice when another supplier let us down which could have resulted in very serious disruption.

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Enzyme Supplies’ has been supplying us with a range of enzymes for over 3 years…consistently high quality…competitive… efficient…will continue to use Enzyme Supplies as it’s supplier of enzyme

We changed from one of your competitors and now purchase our enzymes from Enzyme Supplies. The improvement in lead time has been fantastic and your service is excellent. Any question has been answered promptly and with a deep understanding of the material you provide, this did not happen with our previous supplier. I have already told you that I intend our relationship will be a long term one’.

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